hum0053 Release Note
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DNAs extracted from established mesothelioma cell lines from Japanese malignant pleural mesothelioma patients were sequenced using 10X Genomics sequencing technologies. Fastq files and vcf/bed files for structural variants are provided.
DNAs were extracted from
(1) surgical specimens or established mesothelioma cell lines from Japanese malignant pleural mesothelioma patients
(2) peripheral blood cells of the same patients as controls.
DNA fragments containing whole coding exons were concentrated using SureSelect Human All Exon V4 or V5 plus lncRNA (Agilent Technologies) followed by 100 bp paired-end sequencing with HiSeq 2000/2500 (Illumina). Fastq files were mapped onto a reference genome hg19 with BWA and NovoAlign (bam files).