NBDC Research ID: hum0045.v1



Aims: Screening of oncogenic fusion genes in acute lymphoblastic leukemia (ALL) in adolescents and young adults

Methods: RNA-seq by using HiSeq 2000 / 2500

Participants/Materials: 99 Philadelphia chromosome-negative (Ph-) ALLs, 3 Philadelphia chromosome-positive (Ph+) ALLs, 5 cell lines, 8 normal hematopoietic cells

URL: http://mano-lab.umin.jp/english/research.html


Data Set IDType of DataCriteriaRelease Date
JGAS00000000047 NGS (RNA-seq) Controlled-Access (Type I) 2016/03/29

*Release Note

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99 Ph- ALLs

3 Ph+ ALLs,

5 cell lines

8 normal hematopoietic cells

Targets RNA-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2000/2500]
Library Source

[Ph- / + ALL cases]

mRNA extracted from peripheral blood cells or bone marrow aspirates

[cell lines, normal hematopoietic cells]

mRNA extracted from cultured cells

Cell Lines cell lines, normal hematopoietic cells (Takara/Lonza)
Library Construction (kit name) NEBNext Ultra Directional RNA Library Prep Kit for Illumina
Fragmentation Methods Heat treatment
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 104 bp
Japanese Genotype-phenotype Archive Data set ID JGAD00000000047
Total Data Volume 3.29 TB (fastq)
Comments (Policies) NBDC policy & P-DIRECT policy



Principal Investigator: Hiroyuki Mano

Affiliation: The University of Tokyo, Graduate School of Medicine, Department of Cellular Signaling

Project / Group Name:

URL: http://mano-lab.umin.jp/english/research.html

Funds / Grants (Research Project Number):

NameTitleProject Number
Leading Advanced Projects for medical innovation, Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED-LEAP) Projects for exploration of therapeutic targets in cancer 15653406



TitleDOIData Set ID
1 Recurrent DUX4 fusions in B-cell acute lymphoblastic leukemia of adolescents and young adults doi:10.1038/ng.3535 JGAD00000000047


USERS (Controlled-Access Data)

Principal Investigator: Affiliation: Data in Use (Data Set ID)Period of Data Use
Robert Bradley Fred Hutchinson Cancer Research Center, Public Health Science JGAD00000000047 2016/08/04-2016/12/27