NBDC Research ID: hum0203.v1

SUMMARY

Aims: To clarify the genomic feature of Japanese multiple myeloma (MM) patients by comprehensive genetic analyses

Methods: Targeted capture sequencing

Participants/Materials: Japanese MM

Data Set ID	Type of Data	Criteria	Release Date
JGAS000206	NGS (Target Capture)	Controlled Access (Type I)	2020/06/01

* Data users need to apply the Form 2 (Application Form for Using NBDC Human Data) to reach the Controlled Access Data. Learn more

MOLECULAR DATA


Participants/Materials	MM (ICD10: C900): 154 cases primary tumor tissues: 154 samples recurrent tumor tissues: 6 samples peripheral blood mononuclear cells as a normal sample: 23 samples
Targets	Target Capture
Target Loci for Capture Methods	340 genes + immunoglobulin variable regions (IGH, IGK, IGL)
Platform	Illumina [HiSeq 2500]
Library Source	DNAs extracted from frozen bone marrow CD138 positive cells isolated from bone marrow aspirates and peripheral blood mononuclear cells
Cell Lines	-
Library Construction (kit name)	SureSelect custom kit
Fragmentation Methods	Ultrasonic fragmentation (Covaris ME220)
Spot Type	Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	100-200 bp
Japanese Genotype-phenotype Archive Data set ID	JGAD000291
Total Data Volume	255 GB Data files: 366 (fastq) Analysis files: 1 (vcf [ref: hg19])
Comments (Policies)	NBDC policy

Principal Investigator: Keizo Horibe

Affiliation: Clinical Researc Center, Nagoya Medical Center

Project / Group Name: Program for an Integrated Database of Clinical and Genomic Information

URL： https://www.amed.go.jp/en/program/list/14/01/006.html

Funds / Grants (Research Project Number):

Name	Title	Project Number
Program for an Integrated Database of Clinical and Genomic Information, Japan Agency for Medical Research and Development (AMED)	Establishment of an integrated database for clinical and genomic data in cancer	16kk0205005h0001

	Title	DOI	Data Set ID
1	Genomic analysis of multiple myeloma using targeted capture sequencing in the Japanese cohort	doi: 10.1111/bjh.16720	JGAD000291
2

Principal Investigator:	Affiliation:	Data in Use (Data Set ID)	Period of Data Use