NBDC Research ID: hum0203.v1
SUMMARY
Aims: To clarify the genomic feature of Japanese multiple myeloma (MM) patients by comprehensive genetic analyses
Methods: Targeted capture sequencing
Participants/Materials: Japanese MM
Data Set ID | Type of Data | Criteria | Release Date |
---|---|---|---|
JGAS000206 | NGS (Target Capture) | Controlled Access (Type I) | 2020/06/01 |
* Data users need to apply the Form 2 (Application Form for Using NBDC Human Data) to reach the Controlled Access Data. Learn more
MOLECULAR DATA
Participants/Materials |
MM (ICD10: C900): 154 cases primary tumor tissues: 154 samples recurrent tumor tissues: 6 samples peripheral blood mononuclear cells as a normal sample: 23 samples |
Targets | Target Capture |
Target Loci for Capture Methods | 340 genes + immunoglobulin variable regions (IGH, IGK, IGL) |
Platform | Illumina [HiSeq 2500] |
Library Source | DNAs extracted from frozen bone marrow CD138 positive cells isolated from bone marrow aspirates and peripheral blood mononuclear cells |
Cell Lines | - |
Library Construction (kit name) | SureSelect custom kit |
Fragmentation Methods | Ultrasonic fragmentation (Covaris ME220) |
Spot Type | Paired-end |
Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100-200 bp |
Japanese Genotype-phenotype Archive Data set ID | JGAD000291 |
Total Data Volume |
255 GB Data files: 366 (fastq) Analysis files: 1 (vcf [ref: hg19]) |
Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Keizo Horibe
Affiliation: Clinical Researc Center, Nagoya Medical Center
Project / Group Name: Program for an Integrated Database of Clinical and Genomic Information
URL: https://www.amed.go.jp/en/program/list/14/01/006.html
Funds / Grants (Research Project Number):
Name | Title | Project Number |
---|---|---|
Program for an Integrated Database of Clinical and Genomic Information, Japan Agency for Medical Research and Development (AMED) | Establishment of an integrated database for clinical and genomic data in cancer | 16kk0205005h0001 |
PUBLICATIONS
Title | DOI | Data Set ID | |
---|---|---|---|
1 | Genomic analysis of multiple myeloma using targeted capture sequencing in the Japanese cohort | doi: 10.1111/bjh.16720 | JGAD000291 |
2 |
USRES (Controlled-Access Data)
Principal Investigator: | Affiliation: | Data in Use (Data Set ID) | Period of Data Use |
---|---|---|---|