NBDC Research ID: hum0146.v1



Aims: To investigate DNA methylation change in Silver-Russell Syndrome (SRS) patients with assisted reproduction technologies (ART)

Methods: Reduced Representation Bisulfite Sequencing (RRBS)

Participants/Materials: SRS patients born after assisted reproduction technologies (ART), spontaneously conceived SRS patients, and spontaneously conceived children

URL: http://www.med.tohoku.ac.jp/english/about/laboratory/101.html


Data Set IDType of DataCriteriaRelease Date
DRA007187 NGS (RRBS) Un-restricted Access 2019/02/19

*Release Note





5 SRS patients born after ART (ICD10: Q871)

5 spontaneously conceived SRS patients (ICD10: Q871)

10 spontaneously conceived children

Targets RRBS
Target Loci for Capture Methods -
Platform Illumina [HiSeq2500]
Library Source DNA extracted from peripheral blood cells
Cell Lines -
Library Construction (kit name) Reduced representation bisulfite sequncing (RRBS)
Fragmentation Methods Restriction enzyme MspI
Spot Type Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 101 bp (includes adaptors)
Calculation for Methylation Rate Bismark methylation extractor (v.0.9.0)
DDBJ Sequence Read Archive ID DRA007187
Total Data Volume 38.5 GB (fastq)
Comments (Policies) NBDC policy

* When the research results including the data which were downloaded from DRA database, are published or presented somewhere, the data user must refer the papers which are originally reported, or include in the acknowledgment.Example



Principal Investigator: Takahiro Arima

Affiliation: Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
Practical Research Project for Rare / Intractable Diseases, Japan Agency for Medical Research and Development (AMED) Molecular diagnosis of congenital imprinting disorders and associated with assisted reproductive technology (ART) JP17ek0109132h003
KAKENHI Grant-in-Aid for Scientific Research (B) Epigenetic cohort study in the ART-derived children 17H04335



TitleDOIData Set ID
1 Association of four imprinting disorders and ART. doi: 10.1186/s13148-019-0623-3 DRA007187