NBDC Research ID: hum0139.v1

 

SUMMARY

Aims: To address how status of KRAS in iPS cells impacts upon self-renewal and differentiation propensity

Methods: Roles of KRAS on stemness were investigated in the context of induced pluripotent stem cells (iPSCs). KRAS mutant (G13C/WT) and wild-type isogenic (WT/WT) iPSCs from a Ras-associated autoimmune leukoproliferative disorder (RALD) patient were used. Retention of self-renewal and capacity for neuronal differentiation were compared.

Participants/Materials: A RALD patient-derived iPSC clones (one with KRAS G13C mutation and the other one with no mutation derived from the same patient).

 

Data Set IDType of DataCriteriaRelease Date
JGAS00000000136 NGS (Exome) Controlled Access (Type I) 2018/07/10

*Release Note 

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MOLECULAR DATA

JGAS00000000136

Participants/Materials

iPSCs from 1 RALD patient (ICD10: D899)

      KRAS mutant (G13C/WT) iPSCs: 1 sample

      wild-type isogenic (WT/WT) iPSCs: 1 sample

Targets Exome
Target Loci for Capture Methods -
Platform Illumina [HiSeq 1500]
Library Source DNAs extracted from each iPSC
Cell Lines -
Library Construction (kit name) SureSelect Human All Exon V5
Fragmentation Methods SureSelect QXT Human All Exon V5: Enzymatic Shearing (transposase-based library prep)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 100 bp x 2
Japanese Genotype-phenotype Archive Data set ID JGAD00000000205
Total Data Volume

32 GB

    Data files: 4 (fastq)

    Analysis files: 2 (bam [ref: hg19])

Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Makoto Otsu

Affiliation: Institute of Medical Science, University of Tokyo

Project / Group Name: The program for intractable diseases research utilizing disease-specific iPS cells

Funds / Grants (Research Project Number):

Name Title Project Number
Research Center Network for Realization of Regenerative Medicine The program for intractable diseases research utilizing disease-specific iPS cells JP16bm0609006, JP17bm0804004

 

PUBLICATIONS

TitleDOIData Set ID
1 Status of KRAS in iPS cells impacts upon self-renewal and differentiation propensity. doi: 10.1016/j.stemcr.2018.06.008 JGAD00000000205
2

 

USERS (Controlled-Access Data)

Principal Investigator: Affiliation: Data in Use (Data Set ID)Period of Data Use