NBDC Research ID: hum0125.v1

 

SUMMARY

Aims: Analysis of immune cells in autoimmune diseases

Methods: RNA-seq

Participants/Materials: Lesions from Crohn's disease patients and normal colorectal tissues from colorectal cancer patients

 

Data Set IDType of DataCriteriaRelease Date
JGAS00000000127 NGS (RNA-seq) Controlled Access (Type I) 2018/11/05

*Release Note

* Data users need to apply the Form 2 (Application Form for Using NBDC Human Data) to reach the Controlled Access Data. Learn more

 

MOLECULAR DATA

JGAS00000000127

Participants/Materials

Crohn's disease (ICD10: K50): 3 cases

colorectal cancer: 4 cases (normal tissues)

Targets RNA-seq
Target Loci for Capture Methods -
Platform Thermo Fisher Scientific [Ion S5]
Library Source RNAs extracted from isolated cells using different markers (CD14-CD11c- / CD14-CD11c- / CD14+CD11c-CD163low) from lesions of Crohn's diseases and normal colorectal tissues of colorectal cancers
Cell Lines -
Library Construction (kit name) KAPA Library Preparation Kit
Fragmentation Methods KAPA Frag
Spot Type Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 150 bp on average
Japanese Genotype-phenotype Archive Data set ID JGAD00000000138
Total Data Volume 86 GB (fastq)
Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Shimon Sakaguchi

Affiliation: Experimental Immunology, IFReC, Osaka University

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
KAKENHI Grant-in-Aid for Scientific Research (A) Analysis of the pathology of inflammatory bowel disease 15H02511
KAKENHI Grant-in-Aid for Young Scientists (B) Mechanism and function analysis of abnormal activation of antigen presenting cells in inflammatory bowel disease 17K16546

 

PUBLICATIONS

TitleDOIData Set ID
1 Innate Myeloid Cell Subset-Specific Gene Expression Patterns in the Human Colon are Altered in Crohn’s Disease Patients doi: 10.1159/000490890 JGAD00000000138
2

 

USRES (Controlled-Access Data)

Principal Investigator: Affiliation: Data in Use (Data Set ID)Period of Data Use