NBDC Research ID: hum0117.v1



Aims: Detecting Neuron-specifc methylation changes in Alzheimer's disease brain

Methods: Illumina Infinium® Human Methylation 450K BeadChip

Participants/Materials: 30 Alzheimer's diseasepatients and 30 healthy controls


Data Set IDType of DataCriteriaRelease Date
JGAS00000000125 Methylation array Controlled Access (Type I) 2017/12/11

*Release Note

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Alzheimer's disease (ICD10:G309): 30 cases

Healthy control: 30 individuals

Targets Methylation array
Target Loci for Capture Methods -
Platform Illumina Infinium® Human Methylation 450K BeadChip
Library Source DNAs extracted from autopsied brain tissue
Cell Lines -
Library Construction (kit name)

Bisulfite Conversion: EZ DNA Methylation™ Kit (Zymo Research)

Methylation analysis: Infinium® Human Methylation 450K BeadChip (Illumina)

Algorithms for Calculating Methylation-rate (software) iScan scanner (Illumina)
Filtering Methods -
Normalization of microarray -
Probe Number 487,557 probes (raw data)
Japanese Genotype-phenotype Archive Data set ID JGAD00000000136
Total Data Volume 1 TB (idat)
Comments (Policies) NBDC policy



Principal Investigator: Atsushi Iwata

Affiliation: The University of Tokyo Hospital

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
KAKENHI Grant-in-Aid for Scientific Research (B) A novel neuro-epigenetic approach to the pathogenic mechanism of the neurodegenerative dementia diseases 16H05319
KAKENHI Grant-in-Aid for Young Scientists (B) Analysis of DNA damage mechanism in the novel cellular and mouse model of Alzheimer’s disease 17K16113
Japan Agency for Medical Research and Development Development of a new disease-modifying therapy and early diagnostic method of Alzheimer’s disease based on a cause of amyloid deposition observed in sporadic type 16dm0107069h0001



TitleDOIData Set ID
1 Neuron-specific methylome analysis reveals epigenetic regulation and tau-related dysfunction of BRCA1 in Alzheimer's disease. doi: 10.1073/pnas.1707151114 JGAD00000000136
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USERS (Controlled-Access Data)

Principal Investigator: Affiliation: Data in Use (Data Set ID)Period of Data Use