NBDC Research ID: hum0096.v1

 

SUMMARY

Aims: To explore genetic alteratoins in pediatric T-cell acute lymphoblastic leukemia (T-ALL), we performed genome wide analysis using next generation sequencing methods.

Methods: We performed whole transcriptome sequencing in 123 pediatric T-ALL for detecting gene fusion and analysing gene expression.

Participants/Materials: 123 pediatric T-ALL patients

 

Data Set IDType of DataCriteriaRelease Date
JGAS00000000090 NGS (RNA-seq) Controlled Access (Type I) 2017/05/25

*Release Note

* Data users need to apply the Form 2 (Application Form for Using NBDC Human Data) to reach the Controlled Access Data. Learn more

 

MOLECULAR DATA

JGAS00000000090

Participants/Materials pediatric T-ALL patients (ICD10: C91.0): 123 cases
Targets RNA-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2500]
Library Source total RNAs extracted from bone marrow aspirates, peripheral blood cells, or lymph nodes of pediatric T-ALL patients
Cell Lines -
Library Construction (kit name)

Illumina TruSeq RNA Library Prep Kit

NEB NEBNext Ultra RNA Library Prep Kit for Illumina

Fragmentation Methods restriction enzyme reaction
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 108 bp
Japanese Genotype-phenotype Archive Data set ID JGAD00000000090
Total Data Volume

927 GB

 Data files : 123 (bam [ref: hg19])

Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Junko Takita

Affiliation: Department of Pediatrics, Graduate School of Medicine, The University of Tokyo

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
Project for Cancer Research and Therapeutic Evolution (P-CREATE) Development for novel therapeutic stratagies of intractable cancers in children and adolescent and young adult using molecular profilings 16cm0106509h001
KAKENHI Grant-in-Aid for Scientific Research (B) Development for novel therapeutic stratagies of intractable pediatric cancers based on the multi-omics analysis 17H04224

 

PUBLICATIONS

TitleDOIData Set ID
1 Recurrent PU.1 (SPI1) fusions in high-risk pediatric T cell acute lymphoblastic leukemia doi: 10.1038/ng.3900 JGAD00000000090
2

 

USRES (Controlled-Access Data)

Principal Investigator: Affiliation: Data in Use (Data Set ID)Period of Data Use
Jinyan HUANG Shanghai Jiao Tong University School of Medicine, State Key Laboratory Of Medical Genomics, Rui-jin Hospital JGAD00000000090 2017/07/19-2018/04/01
BERNARD Olivier Institut Gustave Roussy JGAD00000000090 2017/09/14-2019/12/31