hum0090 Release Note

Research IDRelease DateType of Data
hum0090.v1 2016/11/18 NGS (WGBS, ChIP-seq, RNA-seq)

 

hum0090.v1

NGS (WGBS)

gDNAs extracted from normal human absorptive epithelial cells purified from colorectal partial resection specimens were used for analyses (fastq format files). After library preparation by post-bisulfite adaptor-tagging (PBAT, http://crest-ihec.jp/english/epigenome/index.html) and multiplex sequence was performed with the Illumina HiSeq 2000 sequencer (100 bp, Single-end).

 

NGS (ChIP-seq)

Anti H3K4me3,H3K9me3, H3K27me3, H3K27ac, H3K4me1 and H3K36me3 mouse monoclonal antibodies, kindly provided Dr. Hiroshi Kimura, Tokyo Institute of Technology, were used for immunoprecipitation. ChIP-Seq libraries were sequenced using the HiSeq 2500 (Illumina) platform.

 

NGS (RNA-seq)

Total RNAs extracted from normal human absorptive epithelial cells purified from colorectal partial resection specimens were used for whole transcriptome-sequencing analysis (fastq format files). cDNAs were prepared using the Agilent SureSelect Strand Specific RNA kit and multiplex sequence was performed with the Illumina HiSeq 2000 sequencer (100 bp, Paired-end).

 

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