NBDC Research ID: hum0065.v1
SUMMARY
Aims: This study aimed at establishment of CTOS (cancer tissue-originated spheroid) panel covering intra-patient heterogeneity for colorectal cancer, lung cancer and small cell carcinoma of the cervix. With the panel, changes in preparation, culture, and xenograft were pathologically, biochemically, and molecular biologically monitored.
Methods: Exome analysis by using Illumina HiSeq 2000
Participants/Materials: Colorectal cancer, Lung cancer, Small cell carcinoma of uterine cervix
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000089 | NGS (Exome) | Controlled-access (Type I) | 2018/04/06 |
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MOLECULAR DATA
| Participants/Materials |
colorectal cancer: 8 cases, lung cancer: 2 cases, small cell carcinoma of uterine cervix: 2case 1. blood cells: 12 samples (colorectal cancer: 8 samples, lung cancer: 2 samples, small cell carcinoma of uterine cervix: 2samples) 2. CTOS (primary culture): CTOS derived from patient tumors: 5 samples (colorectal cancer: 2 samples, lung cancer: 2 samples, small cell carcinoma of uterine cervix: 1 sample) 3. CTOS (subculture): a. CTOS prepared from a CTOS (the same as 2)-derived mouse xenograft: 14 samples 1st subculture: 1 sample (colorectal cancer: 1 sample) 2nd subculture: 4 samples (small cell carcinoma of uterine cervix: 1 sample, colorectal cancer: 3 samples) 3rd subculture: 5 samples (colorectal cancer: 3 samples, lung cancer: 2 samples) 5th subculture: 4 samples (colorectal cancer: 2 samples, lung cancer: 2 samples) b. CTOS (the same as 2) cultured in vitro: 3 samples (lung cancer: 2 samples, colorectal cancer: 1 sample) 2nd subculture: 1 sample (lung cancer: 1 sample) 5th subculture: 2 samples (lung cancer: 1 sample, colorectal cancer: 1 sample) |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2000] |
| Library Source | DNAs extracted from blood cells and CTOSs |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Human All Exon V5 + lincRNA |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris, E220) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000089 |
| Total Data Volume |
1 TB Data files : 68 (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Masahiro Inoue
Affiliation: Department of Biochemistry, Osaka International Cancer Institute
URL： http://www.mc.pref.osaka.jp/en/department/biochem/
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT), Japan Agency for Medical Research and Development (AMED) | Development of an innovative drug evaluation system using a shuttle system between patient-derived tumors and an ex vivo assay | JP15cm0106155 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | - |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
