NBDC Research ID: hum0060.v1

SUMMARY

Aims: To find the characteristics of recurrent / metastatic breast cancers by carrying out a "molecular profiling", mainly the exome sequence analysis of cancerous tissues of the primary and recurrence tumors from the same patient with recurrent / metastatic breast cancer.

Methods: Whole exome sequencing by using of Illumina HiSeq 2000

Participants/Materials: Total of 175 normal, primary tumor, and metastasis tumor samples from 65 breast cancer patients

Data Set ID	Type of Data	Criteria	Release Date
JGAS000075	NGS (Exome)	Controlled Access (Type I)	2018/04/03

*Release Note

* Data users need to apply the Form 2 (Application Form for Using NBDC Human Data) to reach the Controlled Access Data. Learn more

MOLECULAR DATA

JGAS000075


Participants/Materials	65 patient with recurrent / metastatic breast cancer
Targets	Exome
Target Loci for Capture Methods	-
Platform	Illumina [HiSeq 2000]
Library Source	DNAs extracted from normal, primary tumor and metastasis tumor tissues
Cell Lines	-
Library Construction (kit name)	SureSelect Human All Exon V5 + lincRNA
Fragmentation Methods	Ultrasonic fragmentation (Covaris E220)
Spot Type	Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	100 bp
Japanese Genotype-phenotype Archive Data set ID	JGAD000075
Total Data Volume	4 TB (fastq [350 files])
Comments (Policies)	NBDC policy

DATA PROVIDER

Principal Investigator: Yoshio Miki

Affiliation: Medical Research Institute, Tokyo Medical and Dental University

Project / Group Name: -

Funds / Grants (Research Project Number):

Name	Title	Project Number
Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT)
JSPS KAKENHI

PUBLICATIONS

	Title	DOI	Data Set ID
1

USRES (Controlled-Access Data)

Principal Investigator:	Affiliation:	Data in Use (Data Set ID)	Period of Data Use
Masaki Mandai	Kyoto University Faculty of Medicene, department of Gynecology and Obstetrics	JGAD000075	2020/03/13-2025/03/31