NBDC Research ID: hum0060.v1



Aims: To find the characteristics of recurrent / metastatic breast cancers by carrying out a "molecular profiling", mainly the exome sequence analysis of cancerous tissues of the primary and recurrence tumors from the same patient with recurrent / metastatic breast cancer.

Methods: Whole exome sequencing by using of Illumina HiSeq 2000

Participants/Materials: Total of 175 normal, primary tumor, and metastasis tumor samples from 65 breast cancer patients


Data Set IDType of DataCriteriaRelease Date
NGS (Exome) Controlled Access (Type I) 2018/04/03

*Release Note 

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Participants/Materials 65 patient with recurrent / metastatic breast cancer
Targets Exome
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2000]
Library Source DNAs extracted from normal, primary tumor and metastasis tumor tissues
Cell Lines -
Library Construction (kit name) SureSelect Human All Exon V5 + lincRNA
Fragmentation Methods Ultrasonic fragmentation (Covaris E220)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 100 bp
Japanese Genotype-phenotype Archive Data set ID JGAD00000000075
Total Data Volume 4 TB (fastq [350 files])
Comments (Policies) NBDC policy & P-DIRECT policy



Principal Investigator: Yoshio Miki

Affiliation: Medical Research Institute, Tokyo Medical and Dental University

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT)



TitleDOIData Set ID


USRES (Controlled-Access Data)

Principal Investigator: Affiliation: Data in Use (Data Set ID)Period of Data Use