NBDC Research ID: hum0050.v1

 

SUMMARY

Aims: Understand blood transcriptome of HBV positive blood.

Methods: Extract RNA from whole blood and prepare CAGE libraries for deep sequencing

Participants/Materials: HBV (Genotype C) positive blood samples derived from untreated patients (men)

 

Data Set IDType of DataCriteriaRelease Date
JGAS00000000053 NGS (CAGE-seq) Controlled Access (Type I) 2016/09/30

*Release Note 

* Data users need to apply the Form 2 (Application Form for Using NBDC Human Data) to reach the Controlled Access Data. Learn more

 

MOLECULAR DATA

JGAS00000000053

Participants/Materials 8 HBV (Genotype C) positive blood samples derived from untreated patients (men)
Targets CAGE-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2000]
Library Source RNA extracted from whole blood samples
Cell Lines -
Library Construction (kit name) nAnT-iCAGE v2.1
Fragmentation Methods -
Spot Type Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 47 bp
Japanese Genotype-phenotype Archive Data set ID JGAD00000000053
Total Data Volume 3 GB (fastq, bam [ref: hg19])
Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Tomokazu Matsuura

Affiliation: The Jikei University School of Medicine, Rinsho Kensa Igaku Koza

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
Division of Infectious Disease Research, Research Program on Hepatitis, Japan Agency for Medical Research and Development (AMED) Drug Discovery Research for Complete Cure of Chronic Hepatitis Type B Utilizing The Next Generation Life Science Technologies 15fk0310004h0004

 

PUBLICATIONS

TitleDOIData Set ID
1 Single-nucleotide-resolution mapping of HBV promoters in infected human livers and hepatocellular carcinoma doi: 10.1128/JVI.01625-16 JGAS00000000053
2

 

USRES (Controlled-Access Data)

Principal Investigator: Affiliation: Data in Use (Data Set ID)Period of Data Use