NBDC Research ID: hum0033.v1
Aims： Human whole saliva (WS) contains two types of exosomes (exosome I and exosome II) that are small membrane vesicle (30-100 nm in diameter). To clarify the host defense functions of exosomes, we investigated long RNA components (protein-coding RNAs and long non-coding RNAs) of exosome I, exosome II, and WS by next generation sequencing.
Methods： Human whole saliva was collected by discharge. Long RNA components (protein-coding RNA and long non-coding RNA) of exosome I, exosome II, and WS were analyzed by next generation sequencing.
Participants/Materials： Human whole saliva was collected from one Japanese healthy female volunteer with written informed consent.
|Data ID||Type of Data||Criteria||Release Date|
|DRA003516||NGS (RNA-seq)||Un-restricted Access||2015/05/01|
|Participants/Materials||One Japanese healthy female volunteer|
|Target Loci for Capture Methods||-|
|Platform||Illumina [Genome Analyzer IIx]|
|Library Source||Long RNA components (protein-coding RNA and long non-coding RNA) of exosome I, exosome II, and WS|
|Library Construction (kit name)||Isolated RNA samples from exosome I, exosome II, and WS were amplified using the RampUP RNA Amplification Kit (Genisphere, PA, USA). cDNAs were generated using the amplified sense RNA, according to the manufacturer (Illumina Inc., CA, USA). The cDNA fraction in the range of 200–400 nucleotides was extracted and amplified by PCR.|
|Read Length (without Barcodes, Adaptors, Primers, and Linkers)||36-51 bp|
|DDBJ Sequence Read Archive ID||DRA003516|
|Total Data Volume||3.9 GB|
|Comments (Policies)||NBDC policy|
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Principal Investigator: Ryohei Yanoshita
Affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Teikyo Heisei University
Funds / Grants (Research Project Number):
|KAKENHI Grant-in-Aid for Scientific Research (C)||Molecular mechanisms and regulations of immune activation cascades by exosomes expressing aminopeptidases.||25460172|
Japan Science and Technology Agency (JST) Industry-academia Joint Seeds Innovation Project
|Development of a nanocarrier derived from biogenic substances for application to drug development.||-|
|Title||DOI||Data Set ID|
|1||Small RNA transcriptomes of two types of exosomes in human whole saliva determined by next generation sequencing.||doi.org/10.1248/bpb.b12-00607||DRA003516|