NBDC Research ID: hum0022.v1



Aims: To clarify the molecular mechanism underlying malignant transformation of Donor-cell derived leukemia (DCL).

Methods: Whole Exome Sequencing was performed by using of Illumina HiSeq 2000.

Participants/Materials: One DCL patient and one donor


Data Set IDType of DataCriteriaRelease Date
JGAS00000000017 NGS (Exome) Controlled Access (Type I) 2015/2/2

*Release Note

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Participants/Materials One DCL patient (cells were obtained at the points of initial acute myeloid leukemia, remission stage, relapsed stage, and DCL at late stage). One donor (normal peripheral blood)
Targets Exome
Target Loci for Capture Methods


Platform Illumina [HiSeq 2000]
Library Source Genomic DNAs were extracted from leukemic cells from bone marrow aspirate or peripheral blood.
Cell Lines -
Library Construction (kit name) SureSelect Kit (Agilent)
Fragmentation Methods Ultrasonic fragmentation (Covaris S220)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 100 bp
Japanese Genotype-phenotype Archive Data set ID JGAD00000000017
Total Data Volume 500 GB (bam [ref: GRCh37])
Comments (Policies)

NBDC policy & P-DIRECT policy



Principal Investigator: Hitoshi Kiyoi

Affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine

Project / Group Name: Project for Development of Innovative Research on Cancer Therapeutics , Team for Strategic Therapy Design

Funds / Grants (Research Project Number):

NameTitleProject Number

Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT)

Therapy 1 : Establishment of the strategic therapy design based on the development of molecular-targeted drugs

Therapy 1 - ① : Establishment of the optimal remedy based on the leukemia genome study



TitleDOIData Set ID
1 Leukemic evolution of donor-derived cells harboring IDH2 and DNMT3A mutations after allogeneic stem cell transplantation doi:10.1038/leu.2013.278 JGAD00000000017


USERS (Controlled-Access Data)

Principal Investigator: Affiliation: Data in Use (Data Set ID)Period of Data Use
Seishi Ogawa Kyoto University JGAD00000000017 2015/12/22-2016/03/31