hum0017.v1 Release Note
|Research ID||Release Date||Type of Data|
|hum0017.v1||2016/02/22||NGS (Exome, RNA-seq)|
• Exome: 41 samples
Genomic DNAs were extracted from surgically resected PCNSL tissues and paired peripheral blood cells, and subjected to the enrichment of exon fragments by using SureSelect system (Agilent). They were sequenced 100-105 bases from both ends with a HiSeq 2000 system (Illumina) with paired-end mode (bam files).
• RNA-seq: 30 samples
RNAs extracted from surgically resected PCNSL tissues were used for RNA-seq (fastq).
RNA liberally builded using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) and read by Illumina HiSeq 2000 (paired-end: 100 bp).