NBDC Research ID: hum0011.v1

SUMMARY

Aims: To clarify integrated HBV sequences in the human genome.

Methods: Target enrichment sysem-based next generation sequencer

Participants/Materials: PLC/PRF/5 cell, HepG22215 cell, and HB-HCC primary tissues

Dataset ID	Type of Data	Criteria	Release Date
JGAS000015	NGS (Target Capture)	Controlled-access (Type I)	2015/10/07

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MOLECULAR DATA


Participants/Materials	1 PLC/PRF/5 cell 1 HepG22215 cell 8 HB-HCC primary tissues
Targets	Target Capture
Target Loci for Capture Methods	hepatitis B viral integrants with flanking human genomic sequences
Platform	Roche GS FLX Titanium
Library Source	HB-HCC primary tissues
Cell Lines	PLC/PRF/5 cell [hepatocellular carcinoma cell line (producing HBsAg)] HepG22215 cell [hepatoblastoma cell line (contain an integrated copy of the HBV genome)]
Library Construction (kit name)	custom
Fragmentation Methods	Ultrasonic fragmentation (Covaris)
Spot Type	GS Run Processor
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	321.10 bp
Japanese Genotype-phenotype Archive Dataset ID	JGAD000015
Total Data Volume	1.84 GB
Comments (Policies)	NBDC policy

Principal Investigator: Hiroyuki Yamamoto

Affiliation: St. Marianna University School of Medicine, Division of Gastroenterology and Hepatology

Project / Group Name:

Funds / Grants (Research Project Number):

Name	Title	Project Number
KAKENHI Grant-in-Aid for Scientific Research (C)	Sequence analysis at HBV integrants and flanking host genomes	23590964

	Title	DOI	Dataset ID
1	DNA methylation at hepatitis B viral integrants is associated with methylation at flanking human genomic sequences.	doi: 10.1101/gr.175240.114.	JGAD000015
2

Principal Investigator:	Affiliation:	Data in Use (Dataset ID)	Period of Data Use