NBDC Research ID: hum0007.v1
SUMMARY
Aims: To clarify the mechanism of auto-immune diseases and establish the treatment for auto-immune diseases, through analyzing the expression pattarns of Th1 cells and Th17 cells.
Methods: RNA-Seq by using of AppliedBioSystem SoLiD system
Participants/Materials Total RNAs were extracted from naïve T cells, CXCR3+Th1 cells, CD161+Th17 cells, and activated Th17 cells for one individual
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000005 | NGS (RNA-seq) | Controlled-access (Type I) | 2015/02/12 |
*Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more
MOLECULAR DATA
| Participants/Materials | One Japanese healthy individual（naïve T cells, CXCR3+Th1 cells, CD161+Th17 cells, and activated Th17 cells） |
| Targets | RNA-seq |
| Target Loci for Captur Methods | - |
| Platform | Applied BioSystem [5500 Genetic Analyzer] |
| Library Source |
Total RNAs extracted from naïve T cells, CXCR3+Th1 cells, CD161+Th17 cells, and activated Th17 cells of one individual Th17cells were activated by IL-1β and IL-23 |
| Cell Lines | - |
| Library Construction (kit name) | Applied BioSystem [SoLiD SAGE Kit] |
| Fragmentation Methods | - |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 35 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000005 |
| Total Data Volume | 12.16 GB |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Sanju Iwamoto
Affiliation: Department of Biochemistry, Showa University School of Medicine
Project / Group Name: Research for human Th17 subset
URL: http://www10.showa-u.ac.jp/~biochem/Biochem/Biochem_top.html
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| - |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses. | doi:10.4049/jimmunol.179.3.1449 | JGAD000005 |
| 2 |
USERS (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
| Michiaki Hamada | Faculty of Science and Engineering, Waseda University | Japan | Construction of RNA-targeted Drug Discovery Database | JGAD000005 | 2022/12/26-2025/03/31 |
