NBDC Research ID: hum0020.v1

SUMMARY

Aims: To identify causative genes in amyotrophic lateral sclerosis (ALS).

Methods: Whole exome sequence analysis using Illumina HiSeq 2000.

Participants/Materials: 11 familial ALS patients

Dataset ID	Type of Data	Criteria	Release Date
JGAS000013	NGS (Exome)	Controlled-access (Type I)	2015/01/08

*Release Note

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MOLECULAR DATA

JGAS000013


Participants/Materials	11 familial ALS patients
Targets	Exome
Target Loci for Capture Methods	-
Platform	Illumina [HiSeq 2000]
Library Source	gDNA extracted from peripheral blood cells
Cell Lines	-
Library Construction (kit name)	SureSelect Human All Exon 50 Mb Kit (Agilent)
Fragmentation Methods	Ultrasonic fragmentation (Covaris S220)
Spot Type	Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	100 bp
Japanese Genotype-phenotype Archive Dataset ID	JGAD000013
Total Data Volume	110 GB
Comments (Policies)	NBDC policy

This study was supported by “Genome Science”. “Genome Science” is a part of the “Support Programs for Three Fields in Life Sciences (cancer, genome and brain sciences)” established by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) under the Grant-in-Aid for Scientific Research on Innovative Areas.

DATA PROVIDER

Principal Investigator: Fumiaki Tanaka

Affiliation: Department of Neurology and Stroke Medicine, Yokohama City University Graduate School of Medicine

Project / Group Name:

Funds / Grants (Research Project Number):

Name	Title	Project Number
KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas	Elucidation of causative genes and pathogenesis of ALS through personal genome analysis	22129005

PUBLICATIONS

	Title	DOI	Dataset ID
1
2

USERS (Controlled-access Data)

Principal Investigator	Affiliation	Country/Region	Research Title	Data in Use (Dataset ID)	Period of Data Use